S-Adenosylmethionine synthetase (MAT)


1. S-Adenosylmethionine synthetase (MAT) catalyzes the S-adenosylmethionine (SAM) synthesis reaction from ATP and L-Met.




2. Crystal structure S-adenosylmethionine Synthetase complexes with various molecules and ions were determined.



S-Adenosylmethionine Synthetase with AMPPNP and L-Met.



3. This enzyme has a knot in the polypeptide.




4. Catalytic Mechanism of MAT based on the crystal structures and site-directed mutagenesis.





Takusagawa, F., Kamitori, S., Misaki, S. & Markham, G. D. (1996). Crystal Structure of S-Adenosylmethionine Synthetase. J. Biol. Chem. 271, 136-147.

Takusagawa, F., Kamitori, S. & Markham, G.D. (1996). Structure and Function of S-Adenosylmethionine Synthetase: Crystal Structure of S-Adenosylmethionine Synthetase with ADP, Br-ADP, PPi at 2.8 Resolution. Biochemistry 35, 2586-2596.

Fu, Z., Markham, G. D. & Takusagawa, F. (1996). Flexible Loop in the Structure of S-Adenosylmethionine Synthetase Crystallized in the Tetragonal Crystal System. J. Biomol. Struct. Dyn. 13, 727-739.

Takusagawa, F. & Kamitori, S. (1996). A Real Knot in Protein. J. Am. Chem. Soc. 118, 8945-8946.

Takusagawa, F., Fujioka, M. Spies, A. & Schowen, R.L. (1998). S-Adenosylmethionine (AdoMet)-dependent Methyltransferases in Comprehensive Biological Catalysis: A Mechanistic Reference, Vol I (M. Sinnott, ed.), Academic Press, London, pp. 1-30.

Taylor, J. C., Takusagawa, F. & Markham, G. D. (2002). The active site loop of S-adenosylmethionine synthetase modulates catalytic efficiency. Biochemistry 41, 9358-9369.

Komoto, J., Yamada, T., Takata, Y., Markham, G.D.& Takusagawa, F. (2004). Crystal structure of the S-adenosylmethionine synthetase ternary complex: A novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met. Biochemistry 43, 1821-1831.